ELMO GLOW DISCHARGE SYSTEM

ELMO glow discharge system for EM grids

Your TEM grids ready within seconds.

Hydrophilic or hydrophobic, Negative or Positive charge

A Glow Discharge treatment with a specific gas atmosphere will modify the surface properties of TEM support films or grids in order to optimize the adsorption of the solutions to spread.

TEM carbon support films have a hydrophobic tendency. A glow discharge treatment with air makes the carbon film surface negatively charged and hydrophilic which allows an adsorption of aqueous solutions.

Surface	Charge	Atmosphere
Hydrophilic	Positive	Air (with subsequent treatment Mg)*
Hydrophilic	Negative	Air
Hydrophobic	Positive	Amylamine
Hydrophobic	Negative	Methanol

*Magnesium acetate

Thanks to a glow discharge treatment with amylamine the hydrophobic tendency of the carbon film is kept and the surface is charged positively allowing an adsorption of molecules like nucleic acids.

Negative staining with uranyl acetate and rotary shadowing with platinium of plasmid DNA.

Torus obtained by complex coacervation between the peptide P140 and hyaluronic acid (therapeutic aim).

Nanoparticles obtained by complex coacervation between insuline and PLGA.

Torus obtained by complex coacervation between the peptide P140 and hyaluronic acid (therapeutic aim).

Glow discharge setup for grids preparation

Courtesy : Laboratory of cell biology of Tours (France)

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